Christophe Zimmer, Edouard Bertrand

Thomas Walter, Florian Muller

Thomas Walter

Thomas Walter // The cell cycle consists of four phases and impacts most cellular processes. In imaging assays, the cycle phase can be identified using dedicated cell-cycle markers. However, such markers occupy fluorescent channels that may be needed for other reporters. Here, we propose to address this limitation by inferring the phase from a widely used fluorescent reporter: SiR-DNA.

Laura Cantini // In dynamic biological processes such as development, spatial transcriptomics is revolutionizing the study of the mechanisms underlying spatial organization within tissues. Inferring cell fate trajectories from spatial transcriptomics profiled at several time points has thus emerged as a critical goal, requiring novel computational methods.

Geneviève Almouzni

Hossein Salari, Daniel Jost // Chromosomes are complex biopolymers folded into dynamic loops via a loop extrusion process and may experience various mechanical forces in vivo. We develop a force-dependent model of chromatin loop extrusion and investigate its mechanical consequences on chromosome organization using simulations and analytical theory. We show that loop extrusion alters the force–extension behavior of DNA in a non-monotonic manner: extrusion stiffens the chain at low forces but softens it at intermediate and high forces.

Marcelo Nollmann // A key output of the NIH-Common Fund 4D Nucleome (4DN) project 1,2 is the open publication of datasets related to the structure of the human cell nucleus and the genome. Recent years have seen a rapid expansion of multiplexed Fluorescence In Situ Hybridization (FISH) or FISH-omics methods, which quantify the spatial organization of chromatin in single cells, sometimes together with RNA and protein measurements, and provide an expanded understanding of how 3D higher-order chromosome structure relates to transcriptional activity and cell development in both health and disease.

Mieux détecter les pathologies avec le programme de recherche Cell-ID.

In eukaryotes, DNA replication constitutes a complex process whereby multiple origins are stochastically fired, and from which the replication machinery proceeds along chromosomes to achieve the faithful synthesis…

In the past decade, remarkable progress in cancer medicine has been achieved by the development of treatments that target DNA sequence variants…

Cancer cells are highly plastic, allowing them to adapt to changing conditions. Genes related to basic cellular processes evolved in ancient species, while more specialized genes appeared later with multicellularity (metazoan genes) or even after mammals evolved…